AREG Rekombinanter Antikörper

AREG Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250996F2

Kat-Nr. : 86387-1-PBS

Synonyme

Amphiregulin, AR, AREGB, Colorectum cell-derived growth factor, CRDGF

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  Sandwich ELISA standard curve of MP02406-2

  Sandwich ELISA standard curve of MP02406-2, Human Amphiregulin Recombinant Matched Antibody Pair - PBS only. 86387-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4569. 86387-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP02406-1

  MCF-7 human breast cancer cells were cultured stimulated with PMA for 3 days. An aliquot of the culture supernatant was removed, assayed for human Amphiregulin, and measured 96.4 ng/mL.

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  Sample test of MP02406-2

  Urine of six individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 7.4 ng/mL with a range of 2.3-12.5 ng/mL.

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  Sample test of MP02406-2

  Saliva of six individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 95.9 ng/mL with a range of 22.9-168.9 ng/mL.

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  Sample test of MP02406-2

  MDA-MB-453 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Amphiregulin, and measured 915.6 pg/mL.

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  Sample test of MP02406-2

  The mean Amphiregulin concentration was determined to be 378.9 ng/mL in MDA-MB-453 cell extract based on a 1.8 mg/mL extract load.

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  Sample test of MP02406-2

  Plasma of eight individual healthy human donors was measured. The Amphiregulin concentration of detected samples was determined to be 15.22 pg/mL with a range of 6.47-20.52 pg/mL.

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  Cytometric bead array standard curve of MP02406-1

  Cytometric bead array standard curve of MP02406-1, Amphiregulin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86387-2-PBS. Detection antibody: 86387-1-PBS. Standard: Eg4569. Range: 0.781-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86387-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test AREG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AREG fusion protein Eg4569
• Vollständiger Name: amphiregulin
• Berechnetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: NM_001657.3
• Gene symbol: Amphiregulin/AREG
• Gene ID (NCBI): 374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.