ARF4 Polyklonaler Antikörper

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• KD/KO Validated

ARF4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Kat-Nr. : 11673-1-AP

Synonyme

ADP ribosylation factor 4, ARF2, ARF4

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Galerie der Validierungsdaten

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  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11673-1-AP

  WB result of ARF4 antibody (11673-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

• 

  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11673-1-AP

  IP result of anti-ARF4(IP:11673-1-AP, 4ug; Detection:11673-1-AP 1:3000) with HepG2 cells lysate 1080 ug.

• 

  IP experiment of mouse brain using 11673-1-AP

  IP Result of anti-ARF4 (IP:11673-1-AP, 3ug; Detection:11673-1-AP 1:600) with mouse brain tissue lysate 5200ug.

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  IHC staining of human breast cancer using 11673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11673-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11673-1-AP (ARF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 11673-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (11673-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Produktinformation

11673-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ARF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARF4 fusion protein Ag2272
• Vollständiger Name: ADP-ribosylation factor 4
• Berechnetes Molekulargewicht: 180 aa, 21 kDa
• Beobachtetes Molekulargewicht: 16 kDa, 21 kDa
• GenBank-Zugangsnummer: BC003364
• Gene symbol: ARF4
• Gene ID (NCBI): 378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.; Authors - Reiling Jan H JH
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim
human	WB	Proc Natl Acad Sci U S A; Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity.; Authors - Matteo Pasetto ; KD Validated
human	WB	Cell Rep; KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum.; Authors - Ming Yuan Li
human	WB	Oncogene; A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.; Authors - Breege V Howley

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