ARF6 Monoklonaler Antikörper

ARF6 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H5H1

Kat-Nr. : 68924-1-PBS

Synonyme

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

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  WB analysis of LNCaP using 68924-1-Ig (same clone as 68924-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68924-1-Ig (same clone as 68924-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 68924-1-Ig (same clone as 68924-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68924-1-Ig (same clone as 68924-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68924-1-PBS bindet in WB, ELISA ARF6 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARF6 fusion protein Ag12642
• Vollständiger Name: ADP-ribosylation factor 6
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC002952
• Gene symbol: ARF6
• Gene ID (NCBI): 382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.