ARFGAP2 Polyklonaler Antikörper

ARFGAP2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16519-1-AP

Synonyme

ARF GAP 2, ARFGAP2, IRZ, Nbla10535, Zfp289, Zinc finger protein 289, ZNF289

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 16519-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 16519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16519-1-AP bindet in WB, IF,ELISA ARFGAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARFGAP2 fusion protein Ag9740
• Vollständiger Name: ADP-ribosylation factor GTPase activating protein 2
• Berechnetes Molekulargewicht: 521 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: BC030148
• Gene symbol: ARFGAP2
• Gene ID (NCBI): 84364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.