ARFGAP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARFGAP3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15293-1-AP

Synonyme

ARF GAP 3, ARFGAP1, ARFGAP3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15293-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15293-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15293-1-AP

  IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug.

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of A431 using 15293-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  IF Staining of HepG2 using 15293-1-AP

  Immunofluorescent analysis of HepG2 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

15293-1-AP bindet in WB, IP, IHC, IF, ELISA ARFGAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARFGAP3 fusion protein Ag7536
• Vollständiger Name: ADP-ribosylation factor GTPase activating protein 3
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-66 kDa
• GenBank-Zugangsnummer: BC005122
• Gene symbol: ARFGAP3
• Gene ID (NCBI): 26286
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.

Publikationen

Species	Application	Title
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue ; KD Validated
human	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue
mouse	IF	Cell Signal; ArfGAP3 regulates vesicle transport and glucose uptake in myoblasts; Authors - Suting Li