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ARFGAP3 Polyklonaler Antikörper

ARFGAP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15293-1-PBS

Synonyme

ARFGAP1, ARF GAP 3, ADP-ribosylation factor GTPase-activating protein 3

Filter:
  • Western Blot (WB) analysis of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of mouse brain using 15293-1-AP (same clone as 15293-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of Jurkat using 15293-1-AP (same clone as 15293-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IP experiment of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15293-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ARFGAP3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ARFGAP3 fusion protein Ag7536
Vollständiger Name ADP-ribosylation factor GTPase activating protein 3
Berechnetes Molekulargewicht 57 kDa
Beobachtetes Molekulargewicht 57-66 kDa
GenBank-ZugangsnummerBC005122
Gene symbol ARFGAP3
Gene ID (NCBI) 26286
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.