ARFGEF2 Polyklonaler Antikörper

ARFGEF2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31277-1-PBS

Synonyme

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2

---

• 

  WB analysis using 31277-1-AP (same clone as 31277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31277-1-AP (ARFGEF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

31277-1-PBS bindet in WB, IHC, Indirect ELISA ARFGEF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARFGEF2 fusion protein Ag35396
• Vollständiger Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Beobachtetes Molekulargewicht: 202 kDa
• GenBank-Zugangsnummer: BC050449
• Gene symbol: ARFGEF2
• Gene ID (NCBI): 10564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.