ARFGEF2 Rekombinanter Antikörper

ARFGEF2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241538H5

Kat-Nr. : 84296-4-PBS

Synonyme

BIG2, 241538H5, ARFGEP2, Brefeldin A inhibited GEP 2

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  WB analysis using 84296-4-RR (same clone as 84296-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01199-3

  Cytometric bead array standard curve of MP01199-3, ARFGEF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84296-5-PBS. Detection antibody: 84296-4-PBS. Standard: Ag35396. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84296-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

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Geprüfte Anwendungen

Produktinformation

84296-4-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ARFGEF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARFGEF2 fusion protein Ag35396
• Vollständiger Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Beobachtetes Molekulargewicht: 202 kDa
• GenBank-Zugangsnummer: BC050449
• Gene symbol: ARFGEF2
• Gene ID (NCBI): 10564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.