Arginase-1 Polyklonaler Antikörper

Arginase-1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(557)

Rezensionen (1)

Kat-Nr. : 16001-1-AP

Synonyme

ARG1, Arginase 1, liver Arginase, ARG 1, Arg-1

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  WB analysis using 16001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16001-1-AP (ARG1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours; mouse liver tissue and rat liver tissue are positive, other cells express no or low ARG1 under normal condition.

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  WB analysis using 16001-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16001-1-AP (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16001-1-AP

  IP result of anti-Arginase-1 (IP:16001-1-AP, 4ug; Detection:16001-1-AP 1:40000) with mouse liver tissue lysate 1960 ug.

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  IHC staining of human liver cancer using 16001-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16001-1-AP (Arginase-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16001-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16001-1-AP (Arginase-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16001-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16001-1-AP (Arginase-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 16001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Arginase-1 antibody (16001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 16001-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (16001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16001-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (16001-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 383 publications below
• IHC: See 75 publications below
• IF: See 216 publications below
• CoIP: See 1 publications below

Produktinformation

16001-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA Arginase-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Arginase-1 fusion protein Ag8595
• Vollständiger Name: arginase, liver
• Berechnetes Molekulargewicht: 236aa,25 kDa; 322aa,35 kDa
• Beobachtetes Molekulargewicht: 35-36 kDa
• GenBank-Zugangsnummer: BC005321
• Gene symbol: Arginase-1
• Gene ID (NCBI): 383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exist as a homotrimer and has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 is mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses anti-inflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

Publikationen

Species	Application	Title
mouse	IF	Adv Mater; Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects; Authors - Congyang Mao
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
human	IF	Adv Sci (Weinh); Material Stiffness in Cooperation with Macrophage Paracrine Signals Determines the Tenogenic Differentiation of Mesenchymal Stem Cells; Authors - Renwang Sheng
mouse	IF	Biomaterials; Implantation of MSC spheroid-derived 3D decellularized ECM enriched with the MSC secretome ameliorates traumatic brain injury and promotes brain repair; Authors - Grace H Chen
rat,mouse	IHC,IF	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
mouse	IF	Theranostics; Multifunctionally disordered TiO2 nanoneedles prevent periprosthetic infection and enhance osteointegration by killing bacteria and modulating the osteoimmune microenvironment; Authors - Yangmengfan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Debanjan (Verified Customer) (12-23-2019)

This antibody performs very well for immunohistochemistry from human skin samples

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human skin Biopsy

Arginase-1 Antibody Immunofluorescence, validation (1:50 dilution) in Human skin Biopsy (Cat no:16001-1-AP)