- Featured Product
- KD/KO Validated
Arginase-1 Monoklonaler Antikörper
Arginase-1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Kaninchen, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IF-P, IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
5D6D12
Kat-Nr. : 66129-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Hausschwein-Lebergewebe, Mauslebergewebe, Kaninchenlebergewebe, Rattenlebergewebe |
| Erfolgreiche IP | Mauslebergewebe |
| Erfolgreiche Detektion in IHC | Mauslebergewebe, humanes Lebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | Mauslebergewebe |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, Mauslebergewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:2000-1:5000 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 86 publications below |
| IHC | See 8 publications below |
| IF | See 53 publications below |
Produktinformation
66129-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Arginase-1 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Kaninchen, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Arginase-1 fusion protein Ag8810 |
| Vollständiger Name | arginase, liver |
| Berechnetes Molekulargewicht | 236aa,25 kDa; 322aa,35 kDa |
| Beobachtetes Molekulargewicht | 36 kDa |
| GenBank-Zugangsnummer | BC005321 |
| Gene symbol | Arginase-1 |
| Gene ID (NCBI) | 383 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Arginase-1 antibody 66129-1-Ig | Protokoll herunterladen |
| IHC protocol for Arginase-1 antibody 66129-1-Ig | Protokoll herunterladenl |
| IF protocol for Arginase-1 antibody 66129-1-Ig | Protokoll herunterladen |
| IP protocol for Arginase-1 antibody 66129-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. | ||
Bioact Mater 3D printing of interferon γ-preconditioned NSC-derived exosomes/collagen/chitosan biological scaffolds for neurological recovery after TBI | ||
J Control Release Gut commensal bacteria Parabacteroides goldsteinii-derived outer membrane vesicles suppress skin inflammation in psoriasis | ||
J Nanobiotechnology Inflammatory memory-activated biomimetic nanovesicles regulate neutrophil plasticity and metabolic reprogramming for rapid diabetic wound healing via targeting miR-193a-5p/TLR4/JNK/P38 MAPK pathways | ||
Adv Healthc Mater A Smart MMP-9-responsive Hydrogel Releasing M2 Macrophage-derived Exosomes for Diabetic Wound Healing | ||
J Neuroinflammation Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marion (Verified Customer) (11-24-2024) | DAPI (blue) + Iba1 (Red) + Arg1 (green) Arg1 colocalizes with Iba1 staining M2-type microglia in this model of AD. We are satisfied with the results.
 |
