Arginase-1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Arginase-1 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

58

CloneNo.

5D6D12

Kat-Nr. : 66129-1-Ig

Synonyme

ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 66129-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66129-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66129-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66129-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66129-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66129-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat liver using 66129-1-Ig

  IP result of anti-ARG1 (IP:66129-1-Ig, 5ug; Detection:66129-1-Ig 1:1000) with rat liver tissue lysate 5520 ug.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66129-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, Hausschwein-Lebergewebe, Mauslebergewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 4 publications below
• IF: See 23 publications below

Produktinformation

66129-1-Ig bindet in WB, IP, IHC, IF, ELISA Arginase-1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Arginase-1 fusion protein Ag8810
• Vollständiger Name: arginase, liver
• Berechnetes Molekulargewicht: 236aa,25 kDa; 322aa,35 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC005321
• Gene symbol: ARG1
• Gene ID (NCBI): 383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

Publikationen

Species	Application	Title
human,mouse	WB,IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
	WB	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
mouse	WB,IHC,IF	Carbohydr Polym; Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism; Authors - Lu Li
mouse	WB	Int J Nanomedicine; Rosuvastatin Nanomicelles Target Neuroinflammation and Improve Neurological Deficit in a Mouse Model of Intracerebral Hemorrhage.; Authors - Liu Zi
	IF	NPJ Parkinsons Dis; Melatonin ameliorates Parkinson's disease via regulating microglia polarization in a RORα-dependent pathway.; Authors - Jingwen Li