Arginase-1 Monoklonaler Antikörper

Arginase-1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

2

CloneNo.

5D6D12

Kat-Nr. : CL488-66129

Synonyme

ARG1, Arginase 1, arginase, liver, Arginase-1, Liver type arginase, Type I arginase

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-66129

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66129 (ARG1 antibody) at dilution of 1:50.

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  IF Staining of HeLa using CL488-66129

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66129 (ARG1 antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL488-66129 bindet in IF Arginase-1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Arginase-1 fusion protein Ag8810
• Vollständiger Name: arginase, liver
• Berechnetes Molekulargewicht: 236aa,25 kDa; 322aa,35 kDa
• GenBank-Zugangsnummer: BC005321
• Gene symbol: ARG1
• Gene ID (NCBI): 383
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
mouse	IF	Clin Cancer Res; 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition.; Authors - Kirsten M Reeves
mouse	IF	Biosci Rep; Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis.; Authors - Weihao Shi