Arginase-2 Polyklonaler Antikörper

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• KD/KO Validated

Arginase-2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14825-1-PBS

Synonyme

ARG2, ARG 2, EC:3.5.3.1, Arginase-2, mitochondrial, arginase, type II

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  WB analysis of Caco-2 using 14825-1-AP (same clone as 14825-1-PBS)

  WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arginase-2 transfected Caco-2 cells. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14825-1-AP (same clone as 14825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14825-1-PBS bindet in WB, IHC, Indirect ELISA Arginase-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Arginase-2 fusion protein Ag6609
• Vollständiger Name: arginase, type II
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC001350
• Gene symbol: ARG2
• Gene ID (NCBI): 384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)