ARH Monoklonaler Antikörper

ARH Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1D1B11

Kat-Nr. : 66932-2-Ig

Synonyme

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

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  WB analysis using 66932-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (ARH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 66932-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (LDLRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 66932-2-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ARH Monoclonal antibody (66932-2-Ig, Clone:1D1B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

66932-2-Ig bindet in WB, FC (Intra), ELISA ARH und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARH fusion protein Ag28608
• Vollständiger Name: low density lipoprotein receptor adaptor protein 1
• Berechnetes Molekulargewicht: 308 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC029770
• Gene symbol: ARH
• Gene ID (NCBI): 26119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Leukocyte immunoglobulin-like receptor B1 (LILRB1) protects human multiple myeloma cells from ferroptosis by maintaining cholesterol homeostasis; Authors - Miao Xian ; KD Validated