ARHGAP23 Polyklonaler Antikörper

ARHGAP23 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31938-1-AP

Synonyme

KIAA1501, Rho GTPase Activating Protein 23, Rho GTPase-activating protein 23, Rho-type GTPase-activating protein 23

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  WB analysis using 31938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31938-1-AP (ARHGAP23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 31938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31938-1-AP bindet in WB, IHC, ELISA ARHGAP23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGAP23 fusion protein Ag36340
• Vollständiger Name: Rho GTPase activating protein 23
• Berechnetes Molekulargewicht: 162kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_001199417
• Gene symbol: ARHGAP23
• Gene ID (NCBI): 57636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP23 (Rho GTPase Activating Protein 23) belongs to the family of Rho GTPase activating proteins. The protein functions as a GTPase-activating factor by converting Rho class GTPases into their inactive GDP-bound state. ARHGAP23 is involved in the Rho GTPase signaling pathway, affecting processes such as cytoskeletal reorganization, cell migration, and cell proliferation. In non-small cell lung cancer, the expression of ARHGAP23 is associated with tumor immune infiltration and prognosis, and its role in tumor development has attracted attention. Mutations in this gene are related to a variety of diseases, including certain types of cancer and neurological disorders.