ARHGAP4 Rekombinanter Antikörper

ARHGAP4 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242995B5

Kat-Nr. : 85648-2-RR

Synonyme

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

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  WB analysis using 85648-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 85648-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85648-2-RR (ARHGAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85648-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85648-2-RR (ARHGAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 85648-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85648-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85648-2-RR bindet in WB, IHC, IF/ICC, ELISA ARHGAP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARHGAP4 fusion protein Ag10091
• Vollständiger Name: Rho GTPase activating protein 4
• Berechnetes Molekulargewicht: 986 aa, 109 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC052303
• Gene symbol: ARHGAP4
• Gene ID (NCBI): 393
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.