ARHGAP5 Polyklonaler Antikörper

ARHGAP5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 55165-1-AP

Synonyme

ARHGAP5, GFI2, p190 B, RhoGAP5

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Galerie der Validierungsdaten

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  WB analysis using 55165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

55165-1-AP bindet in WB, ELISA ARHGAP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Rho GTPase activating protein 5
• Berechnetes Molekulargewicht: 172 kDa
• Beobachtetes Molekulargewicht: 170-190 kDa
• GenBank-Zugangsnummer: NM_001173
• Gene symbol: ARHGAP5
• Gene ID (NCBI): 394
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP5, also named as RHOGAP5 and p190-B, is GTPase-activating protein for Rho family members. It may play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. ARHGAP5 can be directly phosphorylated on a single identified tyrosine residue by the activated INS and IGF-1 receptors. It also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of ARHGAP5 is shown to attenuate signal transducing activity of Rac, Rho and CDC42. The antibody is specific to ARHGAP5.

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis.; Authors - Ruli Li
human	WB	Nat Metab; Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis.; Authors - Camille Arcinas

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

Good, both for WB and IF.By WB, although there are some non-specific bands, it can detect endogenous protein; by IF, PFA fixation, it labels the protein in the Golgi and the nucleus.

• Applications: Western Blot, Immunofluorescence,