ARHGAP9 Polyklonaler Antikörper

ARHGAP9 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15665-1-AP

Synonyme

Rho-type GTPase-activating protein 9, Rho GTPase-activating protein 9, RGL1, 10C

---

• 

  WB analysis of Jurkat using 15665-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15665-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15665-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 15665-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15665-1-AP (ARHGAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15665-1-AP bindet in WB, IF/ICC, ELISA ARHGAP9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGAP9 fusion protein Ag8104
• Vollständiger Name: Rho GTPase activating protein 9
• Berechnetes Molekulargewicht: 750 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 65 kDa
• GenBank-Zugangsnummer: BC006107
• Gene symbol: ARHGAP9
• Gene ID (NCBI): 64333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.

Publikationen

Species	Application	Title
human	WB	Genomics; ARHGAP9 knockdown promotes lung adenocarcinoma metastasis by activating Wnt/β-catenin signaling pathway via suppressing DKK2; Authors - Wenping Song