ARHGDIB Monoklonaler Antikörper

ARHGDIB Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E7B7

Kat-Nr. : 66054-1-PBS

Synonyme

3E7B7, GDIA2, GDID4, Ly GDI, Ly-GDI

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  WB analysis using 66054-1-Ig (same clone as 66054-1-PBS)

  Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66054-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ARHGDIB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARHGDIB fusion protein Ag9091
• Vollständiger Name: Rho GDP dissociation inhibitor (GDI) beta
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009200
• Gene symbol: ARHGDIB
• Gene ID (NCBI): 397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.