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ARHGEF11 Polyklonaler Antikörper

ARHGEF11 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30508-1-AP

Synonyme

ARHGEF11, DKFZp667F1223, GTRAP48, KIAA0380, PDZ RHOGEF

Filter:
  • Western Blot (WB) analysis of various lysates using ARHGEF11 Polyclonal antibody (30508-1-AP)

    WB analysis using 30508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30508-1-AP (ARHGEF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ARHGEF11 Polyclonal antibody (30508-1-AP)

    IHC staining of mouse colon using 30508-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30508-1-AP (ARHGEF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen, HuH-7-Zellen, Maushirngewebe
Erfolgreiche Detektion in IHCMaus-Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

30508-1-AP bindet in WB, IHC, ELISA ARHGEF11 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ARHGEF11 fusion protein Ag33104
Vollständiger Name Rho guanine nucleotide exchange factor (GEF) 11
Berechnetes Molekulargewicht168 kDa
Beobachtetes Molekulargewicht200-220 kDa
Gene symbol ARHGEF11
Gene ID (NCBI) 9826
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ARHGEF11 antibody 30508-1-APProtokoll herunterladen
IHC protocol for ARHGEF11 antibody 30508-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000 - uppermost bands are PDZ

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MSCs
ARHGEF11 Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:30508-1-AP)