ARHGEF11 Rekombinanter Antikörper

ARHGEF11 Rekombinant Antikörper für IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241427A4

Kat-Nr. : 84149-1-PBS

Synonyme

241427A4, GTRAP48, KIAA0380, PDZ RHOGEF

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  Sandwich ELISA standard curve of MP01063-2

  Sandwich ELISA standard curve of MP01063-2, Human ARHGEF11 Recombinant Matched Antibody Pair - PBS only. 84149-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33104. 84149-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP01063-2

  The mean ARHGEF11 concentration was determined to be 4.0 ng/mL in HEK-293T cell extract based on a 3.2 mg/mL extract load.

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  IHC staining of human colon using 84149-1-RR (same clone as 84149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 84149-1-RR (same clone as 84149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 84149-1-RR (same clone as 84149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using ARHGEF11 antibody (84149-1-RR, Clone: 241427A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01063-2

  Cytometric bead array standard curve of MP01063-2, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-3-PBS. Detection antibody: 84149-1-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84149-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84149-1-PBS against Human ARHGEF11 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 84149-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84149-1-RR against Human ARHGEF11 were performed. The affinity constant is 1.09 nM.

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Geprüfte Anwendungen

Produktinformation

84149-1-PBS bindet in IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ARHGEF11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARHGEF11 fusion protein Ag33104
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 11
• Berechnetes Molekulargewicht: 168 kDa
• Gene symbol: ARHGEF11
• Gene ID (NCBI): 9826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF or GTRAP48. ARHGEF11 contains several functional domains, including a PDZ domain and a RhoGEF domain. The PDZ domain allows it to interact with various proteins, such as ZO-1 and plexins, while the RhoGEF domain enables it to activate Rho GTPases. ARHGEF11 is a multifunctional protein that plays crucial roles in cell adhesion, signal transduction, and development. Its dysregulation has been linked to several diseases, making it an important area of research for potential therapeutic applications.