ARHGEF5 Polyklonaler Antikörper

ARHGEF5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11379-1-PBS

Synonyme

Ephexin-3, GEF5, Oncogene TIM, P60, p60 TIM

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  WB analysis of MCF-7 using 11379-1-AP (same clone as 11379-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11379-1-AP (same clone as 11379-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11379-1-AP (same clone as 11379-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 11379-1-AP (same clone as 11379-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11379-1-AP (same clone as 11379-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11379-1-AP (same clone as 11379-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11379-1-AP (same clone as 11379-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)

  IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11379-1-AP (same clone as 11379-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARHGEF5 antibody (11379-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11379-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ARHGEF5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGEF5 fusion protein Ag1892
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 5
• Berechnetes Molekulargewicht: 177 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC010046
• Gene symbol: ARHGEF5
• Gene ID (NCBI): 7984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.