ARID1A Rekombinanter Antikörper

ARID1A Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240690F1

Kat-Nr. : 83733-3-PBS

Synonyme

240690F1, ARID domain-containing protein 1A, AT-rich interactive domain-containing protein 1A, B120, BAF250

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  WB analysis of BxPC-3 using 83733-3-RR (same clone as 83733-3-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  WB analysis using 83733-3-RR (same clone as 83733-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83733-3-RR (same clone as 83733-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83733-3-RR (same clone as 83733-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00714-2

  Cytometric bead array standard curve of MP00714-2, ARID1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83733-3-PBS. Detection antibody: 83733-2-PBS. Standard: Ag30749. Range: 1.25 -80 ng/mL.

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  Affinity and Kinetic Characterization of 83733-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

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Geprüfte Anwendungen

Produktinformation

83733-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA ARID1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARID1A fusion protein Ag30749
• Vollständiger Name: AT rich interactive domain 1A (SWI-like)
• Berechnetes Molekulargewicht: 242 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa
• GenBank-Zugangsnummer: NM_006015
• Gene symbol: ARID1A
• Gene ID (NCBI): 8289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.