ARID1B Rekombinanter Antikörper

ARID1B Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230222A11

Kat-Nr. : 82979-6-PBS

Synonyme

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

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  IF Staining of HeLa using 82979-6-RR (same clone as 82979-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 82979-6-RR (same clone as 82979-6-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82979-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.

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Geprüfte Anwendungen

Produktinformation

82979-6-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA ARID1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARID1B fusion protein Ag30643
• Vollständiger Name: AT rich interactive domain 1B (SWI1-like)
• Berechnetes Molekulargewicht: 236 kDa
• GenBank-Zugangsnummer: NM_017519
• Gene symbol: ARID1B
• Gene ID (NCBI): 57492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.