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ARID3A Monoklonaler Antikörper

ARID3A Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B10C10

Kat-Nr. : 68428-1-PBS

Synonyme

Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A

Filter:
  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ARID3A Monoclonal antibody (68428-1-Ig)

    IF Staining of K-562 using 68428-1-Ig (same clone as 68428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ARID3A antibody (68428-1-Ig, Clone: 2B10C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68428-1-PBS bindet in WB, IF/ICC, Indirect ELISA ARID3A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ARID3A fusion protein Ag7655
Vollständiger Name AT rich interactive domain 3A (BRIGHT-like)
Berechnetes Molekulargewicht 63 kDa
Beobachtetes Molekulargewicht 75 kDa
GenBank-ZugangsnummerBC060828
Gene symbol ARID3A
Gene ID (NCBI) 1820
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).