ARID4A Rekombinanter Antikörper

ARID4A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240436A4

Kat-Nr. : 83489-1-PBS

Synonyme

240436A4, ARID 4A, RBBP 1, RBBP1, RBP 1

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  WB analysis using 83489-1-RR (same clone as 83489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83489-1-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00489-3

  Sandwich ELISA standard curve of MP00489-3, Human ARID4A Recombinant Matched Antibody Pair - PBS only. 83489-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33114. 83489-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00489-3

  The mean ARID4A concentration was determined to be 9.3 ng/mL in DU 145 cell extract based on a 3.2 mg/mL extract load, 18.1 ng/mL in MCF-7 cell extract based on a 3.5 mg/mL extract load.

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  IHC staining of mouse brain using 83489-1-RR (same clone as 83489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83489-1-RR (ARID4A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83489-1-RR (same clone as 83489-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ARID4A antibody (83489-1-RR, Clone: 240436A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83489-1-RR (same clone as 83489-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ARID4A Recombinant antibody (83489-1-RR, Clone:240436A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83489-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83489-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83489-1-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83489-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ARID4A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARID4A fusion protein Ag33114
• Vollständiger Name: AT rich interactive domain 4A (RBP1-like)
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 205 kDa
• GenBank-Zugangsnummer: NM_002892.3
• Gene symbol: ARID4A
• Gene ID (NCBI): 5926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.