ARID4B Monoklonaler Antikörper

ARID4B Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB

Konjugation

Unkonjugiert

CloneNo.

1H3E2

Kat-Nr. : 67384-1-Ig

Synonyme

ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180

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Galerie der Validierungsdaten

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  WB analysis of T-47D using 67384-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-231 using 67384-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, MDA-MB-231-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67384-1-Ig bindet in WB ARID4B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARID4B fusion protein Ag21485
• Vollständiger Name: AT rich interactive domain 4B (RBP1-like)
• Berechnetes Molekulargewicht: 1312 aa, 148 kDa
• GenBank-Zugangsnummer: BC130418
• Gene symbol: ARID4B
• Gene ID (NCBI): 51742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.