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ARIH2 Polyklonaler Antikörper

ARIH2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15006-1-PBS

Synonyme

ARI 2, ARI2, ARI-2, ARIH 2, E3 ubiquitin-protein ligase ARIH2

Filter:
  • Western Blot (WB) analysis of various lysates using ARIH2 Polyclonal antibody (15006-1-AP)

    WB analysis using 15006-1-AP (same clone as 15006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15006-1-AP (ARIH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ARIH2 Polyclonal antibody (15006-1-AP)

    IP experiment of HeLa using 15006-1-AP (same clone as 15006-1-PBS)

    IP result of anti-ARIH2 (IP:15006-1-AP, 4ug; Detection:15006-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARIH2 Polyclonal antibody (15006-1-AP)

    IF Staining of HEK-293 using 15006-1-AP (same clone as 15006-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15006-1-AP (ARIH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

15006-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA ARIH2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ARIH2 fusion protein Ag6507
Vollständiger Name ariadne homolog 2 (Drosophila)
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC000422
Gene symbol ARIH2
Gene ID (NCBI) 10425
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.