ARK5 Polyklonaler Antikörper

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• KD/KO Validated

ARK5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22723-1-PBS

Synonyme

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  WB analysis using 22723-1-AP (same clone as 22723-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 22723-1-AP (same clone as 22723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22723-1-PBS bindet in WB, IHC, Indirect ELISA ARK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARK5 fusion protein Ag18612
• Vollständiger Name: NUAK family, SNF1-like kinase, 1
• Berechnetes Molekulargewicht: 661 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC152462
• Gene symbol: ARK5
• Gene ID (NCBI): 9891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.