ARL13B Monoklonaler Antikörper

ARL13B Monoklonal Antikörper für WB, IF/ICC, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Hund, Maus

Anwendung

WB, IF/ICC, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6C3

Kat-Nr. : 66739-1-PBS

Synonyme

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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  WB analysis of HepG2 using 66739-1-Ig (same clone as 66739-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66739-1-Ig (same clone as 66739-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66739-1-Ig (same clone as 66739-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66739-1-Ig (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66739-1-Ig (same clone as 66739-1-PBS)

  rozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T /cTnT (red, Cat. No 15513-1-AP), CD31/PECAM-1 (magenta), and Arl13B (green, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 66739-1-Ig (same clone as 66739-1-PBS)

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (magenta, Cat. No 15513-1-AP), CD31/PECAM-1 (white), and Arl13B (yellow, Cat. No 66739-1-Ig) with DAPI as a counterstain for visualizing the nucleus (cyan). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to magenta.CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Arl13B stains primary cilia and was visualized with an Alexa Fluor 488 secondary antibody and psedocolored to yellow. The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 66739-1-Ig (same clone as 66739-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL13B antibody (66739-1-Ig, Clone: 1H6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 66739-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66739-1-PBS bindet in WB, IF/ICC, IF-Fro, Indirect ELISA ARL13B und zeigt Reaktivität mit Hausschwein, human, Hund, Maus

• Getestete Reaktivität: Hausschwein, human, Hund, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12031
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).