ARL13B Rekombinanter Antikörper

ARL13B Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H5

Kat-Nr. : 82834-2-PBS

Synonyme

3H5, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1

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  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82834-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82834-2-RR against Human ARL13B were performed. The affinity constant is 1.25 nM.

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Geprüfte Anwendungen

Produktinformation

82834-2-PBS bindet in IF/ICC, Indirect ELISA ARL13B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12015
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.