ARL13B Polyklonaler Antikörper

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• KD/KO Validated

ARL13B Polyklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

Kat-Nr. : CL488-17711

Synonyme

ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1, ARL2-like protein 1

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  IF Staining of mouse pancreas using CL488-17711

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, E-cadherin antibody (20874-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using CL488-17711

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CEP250/CNAP1 antibody (14498-1-AP, orange), SCLT1 antibody (14875-1-AP, Magenta).

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  IF Staining of hTERT-RPE1 using CL488-17711

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-17711) at dilution of 1:200, CoraLite®594 Acetyl-Tubulin (Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of MDCK using CL488-17711

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-17711 (ARL13B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL488-17711 bindet in IF/ICC, IF-P ARL13B und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12015
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). CoraLite488-Conjugated ARL13B antibody can be used to mark the cilia(green). Ex: 488nm, Em: 515nm

Publikationen

Species	Application	Title
human	IF	Mol Oncol; Interferon regulatory factor 4 modulates epigenetic silencing and cancer-critical pathways in melanoma cells; Authors - Ulduz Sobhiafshar
mouse	IF	Neurosci Res; Phenotypic characterization of Cdkl5-knockdown neurons establishes elongated cilia as a functional assay for CDKL5 Deficiency Disorder.; Authors - Alessia Di Nardo