ARL13B Polyklonaler Antikörper

ARL13B Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Guinea Pig / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-30332

Synonyme

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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  IF Staining of hTERT-RPE1 using CL488-30332

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 ARL13B antibody (CL488-30332) at dilution of 1:200, CoraLite®594 Acetyl-Tubulin (Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of MDCK using CL488-30332

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 ARL13B antibody (CL488-30332) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-30332 bindet in IF/ICC ARL13B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Guinea Pig / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12015
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.