ARL2 Polyklonaler Antikörper

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• KD/KO Validated

ARL2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10232-1-AP

Synonyme

ADP ribosylation factor like 2, ARFL2, ARL2

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 10232-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10232-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10232-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 10232-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10232-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10232-1-AP (ARL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10232-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10232-1-AP (ARL2 antibody) at dilution of 1:100 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, Mauslungengewebe, Mausmilzgewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below

Produktinformation

10232-1-AP bindet in WB, IHC, IF, ELISA ARL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL2 fusion protein Ag0334
• Vollständiger Name: ADP-ribosylation factor-like 2
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC002530
• Gene symbol: ARL2
• Gene ID (NCBI): 402
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ADP-ribosylation factor-like 2 (ARL2) is a member of a functionally distinct group of ARF-like genes. ARL2 interacts with the tubulin-specific chaperone protein known as cofactor D. ARL2 downregulates the tubulin GAP activity of cofactor C, D, and E, and inhibits the binding of D to native tubulin.

Publikationen

Species	Application	Title
rat	WB	J Biol Chem; MicroRNA-15b modulates cellular ATP levels and degenerates mitochondria via Arl2 in neonatal rat cardiac myocytes.; Authors - Nishi Hitoo H ; KD Validated
mouse	WB	J Biol Chem; Selective Impairment of a Subset of Ran-GTP-binding Domains of Ran-binding protein 2 (Ranbp2) Suffices to Recapitulate the Degeneration of the Retinal Pigment Epithelium (RPE) Triggered by Ranbp2 Ablation.; Authors - Hemangi Patil
mouse	WB,IF	J Cell Biochem; GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.; Authors - Chun-Xiang Zhou
mouse	WB	Cell Cycle; GTPase-activating protein Elmod2 is essential for meiotic progression in mouse oocytes.; Authors - Chun-Xiang Zhou
human	WB,IF	Invest Ophthalmol Vis Sci; Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.; Authors - Mori Naoki N
mouse	IF	PeerJ; Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.; Authors - Ruichao Li