ARL4C Polyklonaler Antikörper

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• KD/KO Validated

ARL4C Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 10202-1-AP

Synonyme

ADP-ribosylation factor-like protein 4C, ADP-ribosylation factor-like protein 7, ADP-ribosylation factor-like protein LAK, ARL7

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  WB analysis of HUVEC using 10202-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10202-1-AP

  WB result of ARL4C antibody (10202-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARL4C transfected SKOV-3 cells.

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  WB analysis of human brain using 10202-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10202-1-AP (ARL4C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 10202-1-AP

  IP result of anti-ARL4C (IP:10202-1-AP, 4ug; Detection:10202-1-AP 1:500) with HUVEC cells lysate 3600 ug.

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  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10202-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10202-1-AP (ARL4C Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HUVEC using 10202-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARL4C antibody (10202-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  IF Staining of A549 using 10202-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL4C antibody (10202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Produktinformation

10202-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARL4C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL4C fusion protein Ag0246
• Vollständiger Name: ADP-ribosylation factor-like 4C
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC001051
• Gene symbol: ARL4C
• Gene ID (NCBI): 10123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Cell; A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.; Authors - Banaszynski Laura A LA
human	WB	Genome Med; Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer.; Authors - Taotao Sheng ; KD Validated
mouse	WB,IF	Proc Natl Acad Sci U S A; CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis.; Authors - Jisoo S Han ; KD Validated
human	WB,IHC	Front Pharmacol; Identification of biomarkers and potential drug targets in osteoarthritis based on bioinformatics analysis and mendelian randomization; Authors - Feng Cheng
human	WB,IHC	Cancer Sci; ARL4C is associated with initiation and progression of lung adenocarcinoma and represents a therapeutic target.; Authors - Kenji Kimura
human	WB,IF	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen ; KD Validated

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