ARL4D Rekombinanter Antikörper

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• KD/KO Validated

ARL4D Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241503B7

Kat-Nr. : 84163-3-RR

Synonyme

ARF4L, 241503B7, ADP-ribosylation factor-like protein 4D, ADP-ribosylation factor-like protein 4L, ARL6

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  WB analysis using 84163-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84163-3-RR (ARL4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84163-3-RR

  WB result of ARL4D antibody (84163-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARL4D transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 84163-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84163-3-RR against Human ARL4D were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HepG2-Zellen, Maus-Retina-Gewebe, Ratten-Retina-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84163-3-RR bindet in WB, ELISA ARL4D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARL4D fusion protein Ag7369
• Vollständiger Name: ADP-ribosylation factor-like 4D
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: BC000043
• Gene symbol: ARL4D
• Gene ID (NCBI): 379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).