ARL8A Polyklonaler Antikörper

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• KD/KO Validated

ARL8A Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17060-1-AP

Synonyme

GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

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  WB analysis of HeLa using 17060-1-AP

  WB result of ARL8A antibody (17060-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARL8A transfected HeLa cells.

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  WB analysis of mouse brain using 17060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17060-1-AP (ARL8A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17060-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17060-1-AP (ARL8A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17060-1-AP

  IP result of anti-ARL8A (IP:17060-1-AP, 4ug; Detection:17060-1-AP 1:600) with mouse brain tissue lysate 3440ug.

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  IHC staining of human stomach using 17060-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17060-1-AP (ARL8A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

17060-1-AP bindet in WB, IHC, IF, IP, ELISA ARL8A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL8A fusion protein Ag10788
• Vollständiger Name: ADP-ribosylation factor-like 8A
• Berechnetes Molekulargewicht: 186 aa, 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC015408
• Gene symbol: ARL8A
• Gene ID (NCBI): 127829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A has a calculated molecular weight of 21 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
human	WB	Nat Commun; RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.; Authors - Tal Keren-Kaplan
human	WB	Curr Biol; ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1.; Authors - Tal Keren-Kaplan
human,rat	IF	J Cell Biol; Specific KIF1A-adaptor interactions control selective cargo recognition.; Authors - Jessica J A Hummel ; KD Validated
human	WB	J Cell Sci; The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.; Authors - Ziyan Fang ; KD Validated
human		J Cell Biol; Inhibition of endolysosome fusion increases exosome secretion; Authors - Ganesh Vilas Shelke