ARMC8 Polyklonaler Antikörper

ARMC8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (2)

Kat-Nr. : 12653-1-AP

Synonyme

S863-2, S863 2, HSPC056, Armadillo repeat-containing protein 8, armadillo repeat containing 8

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  WB analysis using 12653-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

12653-1-AP bindet in WB, IHC, IP, ELISA ARMC8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARMC8 fusion protein Ag3374
• Vollständiger Name: armadillo repeat containing 8
• Berechnetes Molekulargewicht: 673 aa, 75.5 kDa
• Beobachtetes Molekulargewicht: 75.5 kDa
• GenBank-Zugangsnummer: BC013424
• Gene symbol: ARMC8
• Gene ID (NCBI): 25852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Armadillo repeat-containing protein 8 (Armc8) is a key factor to regulate cell membrane adhesion complex through promoting α-catenin degradation. Armc8 expression was elevated during atypia-to-carcinoma progression and may be associated with cancer development of breast carcinoma (PMID: 25119601).

Publikationen

Species	Application	Title
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB, IHC	J Dermatol Sci; Downregulation of ARMC8 promotes tumorigenesis through activating Wnt/β-catenin pathway and EMT in cutaneous squamous cell carcinomas.; Authors - Xiangzhi Li
human	WB	Dose Response; MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8.; Authors - Chen Zhang ; KD Validated
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi
human	IP	Cell Rep; mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase; Authors - Brittany Q Pham

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (12-31-2021)

This antibody works quite well. There are a few off target bands though, if the samples are loaded too much.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

ARMC8 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:12653-1-AP)

FH

H (Verified Customer) (09-27-2020)

This antibody detects both ARMC8a and b. The signal was good enough to tell which are the right bands immediately.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

ARMC8 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:12653-1-AP)