ARMCX3 Polyklonaler Antikörper

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• KD/KO Validated

ARMCX3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 25705-1-AP

Synonyme

UNQ2517/PRO6007, Protein ALEX3, KIAA0443, BM-017, Armadillo repeat-containing X-linked protein 3

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  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of fetal human brain using 25705-1-AP

  IP result of anti-ARMCX3 (IP:25705-1-AP, 1:500; Detection:25705-1-AP, 1:3000) with human brain lysate, 150 ug. Courtesied by Dr. Mirra and Dr. Manso of University of Barcelona.

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  IHC staining of human ovary cancer using 25705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25705-1-AP (ARMCX3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: fetal human brain
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below

Produktinformation

25705-1-AP bindet in WB, IHC, IP, ELISA ARMCX3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARMCX3 fusion protein Ag22501
• Vollständiger Name: armadillo repeat containing, X-linked 3
• Berechnetes Molekulargewicht: 379 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC005194
• Gene symbol: ARMCX3
• Gene ID (NCBI): 51566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARMCX3, also named as Armadillo repeat-containing X-linked protein 3, is a 379 amino acid protein, which contains 3 ARM repeats. ARMCX3 is a single pass membrane protein belonging to the armadillo repeat family of proteins. ARMCX3 is believed to play a role in embryonic development and tissue maintenance and may also function as a tumor suppressor.

Publikationen

Species	Application	Title
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
	WB	Sci Signal; A mammalian-specific Alex3/Gαq protein complex regulates mitochondrial trafficking, dendritic complexity, and neuronal survival; Authors - Ismael Izquierdo-Villalba ; KO Validated
human	WB	Cancers (Basel); ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity.; Authors - Serena Mirra
mouse	WB	Int J Obes (Lond); The armadillo-repeat containing X-linked protein 3, ARMCX3, is a negative regulator of the browning of adipose tissue associated with obesity.; Authors - Aleix Gavaldà-Navarro
human	WB	Heliyon; ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells; Authors - Quanying Zhou