ARNT/HIF-1 beta Polyklonaler Antikörper

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• KD/KO Validated

ARNT/HIF-1 beta Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 14105-1-AP

Synonyme

ARNT, HIF-1 beta, HIF1B, bHLHe2, Class E basic helix-loop-helix protein 2

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  WB analysis of HeLa using 14105-1-AP

  WB result of ARNT/HIF-1 beta antibody (14105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT,HIF1B transfected HeLa cells.

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  WB analysis of HepG2 using 14105-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14105-1-AP (ARNT/HIF-1 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14105-1-AP

  IP result of anti-ARNT/HIF-1 beta (IP:14105-1-AP, 4ug; Detection:14105-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 14105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14105-1-AP (ARNT/HIF-1 beta Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14105-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ARNT/HIF-1 beta antibody (14105-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

14105-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARNT/HIF-1 beta und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5238
• Vollständiger Name: aryl hydrocarbon receptor nuclear translocator
• Berechnetes Molekulargewicht: 416 aa, 46 kDa, 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC060838
• Gene symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Publikationen

Species	Application	Title
human	WB	Hepatology; PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.; Authors - Liu Yanfeng Y
human	WB,IF	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan
human	WB	Free Radic Biol Med; Sestrin2 inhibits hypoxia-inducible factor-1α accumulation via AMPK-mediated prolyl hydroxylase regulation.; Authors - Kyuhwa Seo
mouse	WB	Cell Immunol; Fine particulate matter (PM2.5) induces inhibitory memory alveolar macrophages through the AhR/IL-33 pathway; Authors - Yanan Liu
mouse	WB	Front Microbiol; Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.; Authors - Zhixiao Sun
mouse	WB	J Biomed Nanotechnol; Fine Particulate Matter (PM2.5) Promoted the Invasion of Lung Cancer Cells via an ARNT2/PP2A/STAT3/MMP2 Pathway.; Authors - Zhongqi Chen