ARNT/HIF-1 beta Monoklonaler Antikörper

ARNT/HIF-1 beta Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3B12C5

Kat-Nr. : 66732-1-PBS

Synonyme

ARNT, HIF-1 beta, HIF1B, 3B12C5, bHLHe2

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  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66732-1-Ig (same clone as 66732-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARNT/HIF-1 beta antibody (66732-1-Ig, Clone: 3B12C5 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66732-1-Ig (same clone as 66732-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66732-1-PBS bindet in WB, IF/ICC, FC (Intra), ELISA ARNT/HIF-1 beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARNT/HIF-1 beta fusion protein Ag5507
• Vollständiger Name: aryl hydrocarbon receptor nuclear translocator
• Berechnetes Molekulargewicht: 416 aa, 46 kDa, 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC060838
• Gene symbol: ARNT/HIF-1 beta
• Gene ID (NCBI): 405
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia