BMAL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BMAL1 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(72)

Kat-Nr. : 14268-1-AP

Synonyme

ARNTL, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

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  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14268-1-AP

  WB result of BMAL1 antibody (14268-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMAL1 transfected HeLa cells.

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  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 14268-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 14268-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14268-1-AP

  IP result of anti-BMAL1 (IP:14268-1-AP, 4ug; Detection:14268-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IF Staining of U-87 MG using 14268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using BMAL1 antibody (14268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of HeLa using 14268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARNTL (14268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, 3T3-L1-Zellen, HeLa-Zellen, MCF-7-Zellen, Mauslebergewebe, NCI-H1299-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: U-87 MG-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 60 publications below
• IHC: See 15 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 5 publications below

Produktinformation

14268-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA BMAL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BMAL1 fusion protein Ag5586
• Vollständiger Name: aryl hydrocarbon receptor nuclear translocator-like
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-75 kDa
• GenBank-Zugangsnummer: BC041129
• Gene symbol: ARNTL
• Gene ID (NCBI): 406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

Publikationen

Species	Application	Title
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KD Validated
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
mouse	WB	J Pineal Res; Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors; Authors - Yao Zuo
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
mouse	IHC	Theranostics; Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.; Authors - Shuai Wang
mouse	WB	Environ Pollut; Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.; Authors - Feifei Shi