ARNTL Rekombinanter Antikörper

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• KD/KO Validated

ARNTL Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250536E10

Kat-Nr. : 86113-1-PBS

Synonyme

BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

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  WB analysis using 86113-1-RR (same clone as 86113-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86113-1-RR (ARNTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  WB result of ARNTL antibody (86113-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNTL transfected HeLa cells. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86113-1-RR (same clone as 86113-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARNTL antibody (86113-1-RR, Clone: 250536E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86113-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ARNTL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARNTL fusion protein Ag5586
• Vollständiger Name: aryl hydrocarbon receptor nuclear translocator-like
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-75 kDa
• GenBank-Zugangsnummer: BC041129
• Gene symbol: ARNTL
• Gene ID (NCBI): 406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).