ARNTL Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

ARNTL Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250536E10

Kat-Nr. : 86113-1-RR

Synonyme

BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

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  WB analysis using 86113-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86113-1-RR (ARNTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 86113-1-RR

  WB result of ARNTL antibody (86113-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNTL transfected HeLa cells.

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  IHC staining of rat liver using 86113-1-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 86113-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86113-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARNTL antibody (86113-1-RR, Clone: 250536E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HT-1080.Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86113-1-RR bindet in WB, IHC, IF/ICC, ELISA ARNTL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ARNTL fusion protein Ag5586
• Vollständiger Name: aryl hydrocarbon receptor nuclear translocator-like
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69-75 kDa
• GenBank-Zugangsnummer: BC041129
• Gene symbol: ARNTL
• Gene ID (NCBI): 406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).