ARP3/ARP3B Polyklonaler Antikörper

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• KD/KO Validated

ARP3/ARP3B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13822-1-PBS

Synonyme

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  WB analysis using 13822-1-AP (same clone as 13822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13822-1-AP (same clone as 13822-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

  IP result of anti-ARP3/ARP3B (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13822-1-AP (same clone as 13822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13822-1-PBS bindet in WB, IHC, IP, Indirect ELISA ARP3/ARP3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARP3/ARP3B fusion protein Ag4922
• Vollständiger Name: ARP3 actin-related protein 3 homolog (yeast)
• Berechnetes Molekulargewicht: 418 aa 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC044590
• Gene symbol: ARP3/ACTR3
• Gene ID (NCBI): 10096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.