ARP3/ARP3B Monoklonaler Antikörper

ARP3/ARP3B Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D12C7

Kat-Nr. : 68439-1-PBS

Synonyme

Actin like protein 3, Actin related protein 3, ACTR3, ARP3

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  WB analysis using 68439-1-Ig (same clone as 68439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68439-1-Ig (ACTR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68439-1-Ig (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 68439-1-Ig (same clone as 68439-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using ARP3/ARP3B antibody (68439-1-Ig, Clone: 1D12C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68439-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68439-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ARP3/ARP3B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARP3/ARP3B fusion protein Ag5017
• Vollständiger Name: ARP3 actin-related protein 3 homolog (yeast)
• Berechnetes Molekulargewicht: 418 aa 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC044590
• Gene symbol: ARP3/ACTR3
• Gene ID (NCBI): 10096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.