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ARP3/ARP3B Monoklonaler Antikörper

ARP3/ARP3B Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D12C7

Kat-Nr. : CL488-68439

Synonyme

ACTR3, ARP3, Actin like protein 3, Actin related protein 3, Actin-like protein 3

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated ARP3/ARP3B Monoclona (CL488-68439)

    IF Staining of HUVEC using CL488-68439

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 ARP3/ARP3B antibody (CL488-68439, Clone: 1D12C7 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68439 bindet in IF/ICC ARP3/ARP3B und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ARP3/ARP3B fusion protein Ag5017
Vollständiger Name ARP3 actin-related protein 3 homolog (yeast)
Berechnetes Molekulargewicht 418 aa 47 kDa
Beobachtetes Molekulargewicht 47 kDa
GenBank-ZugangsnummerBC044590
Gene symbol ARP3/ACTR3
Gene ID (NCBI) 10096
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 ARP3/ARP3B antibody CL488-68439Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen