ARPC2 Monoklonaler Antikörper

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ARPC2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D8E3

Kat-Nr. : 68145-1-PBS

Synonyme

1D8E3, Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC

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  WB analysis using 68145-1-Ig (same clone as 68145-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  WB result of ARPC2 antibody (68145-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARPC2 transfected HeLa cells. This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 68145-1-Ig (same clone as 68145-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68145-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68145-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ARPC2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARPC2 fusion protein Ag7082
• Vollständiger Name: actin related protein 2/3 complex, subunit 2, 34kDa
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000590
• Gene symbol: ARPC2
• Gene ID (NCBI): 10109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.