ARPC5 Polyklonaler Antikörper

ARPC5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16717-1-AP

Synonyme

p16-ARC, p16 Arc, Arp2/3 complex 16 kDa subunit, ARC16, Actin-related protein 2/3 complex subunit 5

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  WB analysis of mouse brain using 16717-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16717-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16717-1-AP

  Rat brain lysates were subjected to SDS PAGE followed by western blot with 16717-1-AP (ARPC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16717-1-AP (ARPC5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16717-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16717-1-AP (ARPC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 16717-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARPC5 antibody (16717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Thymusgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

16717-1-AP bindet in WB, IHC, IF/ICC, ELISA ARPC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC5 fusion protein Ag10144
• Vollständiger Name: actin related protein 2/3 complex, subunit 5, 16kDa
• Berechnetes Molekulargewicht: 154 aa, 17 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC057237
• Gene symbol: ARPC5
• Gene ID (NCBI): 10092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis; Authors - Jiying Hou
human	WB	J Neurosci; The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.; Authors - Avanti Gokhale
human	WB	Pigment Cell Melanoma Res; YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5); Authors - Jason W. Lui
mouse	WB	J Reprod Dev; MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling; Authors - Lanfang Ma
human	IHC	BMC Cancer; ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas; Authors - Yue Ming
human	WB	Heliyon; Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes; Authors - Shi Tai