Arrestin C Polyklonaler Antikörper
Arrestin C Polyklonal Antikörper für WB, IHC, IF, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11100-2-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | humanes Hirngewebe, Maus-Augengewebe, Mausherzgewebe |
Erfolgreiche Detektion in IHC | Maus-Augengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | Retinal organoids, Maus-Augengewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 1 publications below |
IHC | See 5 publications below |
IF | See 1 publications below |
Produktinformation
11100-2-AP bindet in WB, IHC, IF, ELISA Arrestin C und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Arrestin C fusion protein Ag1580 |
Vollständiger Name | arrestin 3, retinal (X-arrestin) |
Berechnetes Molekulargewicht | 43 kDa |
Beobachtetes Molekulargewicht | 43 kDa |
GenBank-Zugangsnummer | BC012096 |
Gene symbol | Arrestin C |
Gene ID (NCBI) | 407 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Arrestin C antibody 11100-2-AP | Protokoll herunterladen |
IHC protocol for Arrestin C antibody 11100-2-AP | Protokoll herunterladenl |
IF protocol for Arrestin C antibody 11100-2-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression. | ||
Cancers (Basel) Clinical, Genomic, and Pharmacological Study of MYCN-Amplified RB1 Wild-Type Metastatic Retinoblastoma. | ||
Int J Mol Sci Tridimensional Retinoblastoma Cultures as Vitreous Seeds Models for Live-Cell Imaging of Chemotherapy Penetration. | ||
Front Mol Neurosci A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e | ||
Pediatr Blood Cancer Immunohistochemical expression of TFF1 is a marker of poor prognosis in retinoblastoma | ||
Invest Ophthalmol Vis Sci Establishment and Comprehensive Characterization of a Novel Preclinical Platform of Metastatic Retinoblastoma for Therapeutic Developments |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alessandro (Verified Customer) (01-19-2023) | great outcome, no unspecific staining
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