ARRDC1 Polyklonaler Antikörper

ARRDC1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21030-1-PBS

Synonyme

Alpha-arrestin 1, arrestin domain containing 1, Arrestin domain-containing protein 1

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  WB analysis using 21030-1-AP (same clone as 21030-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21030-1-AP (ARRDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21030-1-AP (ARRDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21030-1-AP (ARRDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21030-1-PBS bindet in WB, IHC, Indirect ELISA ARRDC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARRDC1 fusion protein Ag15341
• Vollständiger Name: arrestin domain containing 1
• Berechnetes Molekulargewicht: 433 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC032346
• Gene symbol: ARRDC1
• Gene ID (NCBI): 92714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARRDC1, or Arrestin-Domain Containing Protein 1, is a protein that plays a significant role in the formation and release of extracellular vesicles, specifically a subtype known as ARMMs (Arrestin-Domain Containing Protein 1-mediated microvesicles). ARRDC1 is localized to the cytosolic side of the plasma membrane and recruits the ESCRT-I complex protein TSG101 to initiate outward membrane budding. This protein is also involved in the regulation of protein cargo and release of extracellular vesicles, including both exosomes and ectosomes. The absence of ARRDC1 can lead to changes in the protein cargo within these vesicles, indicating its importance in the sorting and packaging process.